Setup Optics

• Stage Directions
• Alignment Procedure
• Alignment Pre-Flight
• Visual Alignment
• Coarse “Beer” Alignment
• Final “Bead” alignment

Stage Directions

The various knobs on the InFlux provide increasingly fine levels of adjustment. In order from "Coarse" to "Finest" the knobs are:

1. Stream Tilt X / Stream Tilt Y
   1. Primarily used to align stream to waste pipe
2. Stream Stage X / Stream Stage Y
   1. Primary use: Align Stream to SSC/Fluor Detector Paths
3. Laser Stage Vertical
   1. Align Laser to SSC/Fluor Detectors
4. FSC Lens X / FSC Lens Z
   1. Align FSC Lens to 488 Laser in the vertical and horizontal planes perpendicular to the direction of laser light.
5. FSC Lens Y
   1. "Focus" the FSC Lens to the 488 Laser
6. Laser Stage Sweep
   1. Fine adjustment of all lasers to the SSC/Fluor Detectors
   2. Optional: Minor adjustments to FSC Lens

Alignment Procedure

• Laser Stages and Tilt
• Laser Systems Reference

Alignment Pre-Flight

• Laser Power
  • Turn on 488
  • Turn on 457
  • (Optional) - Turn on 671
• Systems Check
  • Camera and Monitor check
  • Sortware check
  • Laser power

Visual Alignment

• Tilt and Stage alignment via pinhole and stream cameras

Coarse “Beer” Alignment

• Laser alignment with beer and pinhole camera
• FSC lens alignment with beer

Final “Bead” alignment

• Sortware Setup
• Laser Alignment: 488nm to SSC/Fluorescence detectors
• Laser Alignment: 488nm to FSC
• Laser Alignmwnt: 457nm to Fluorescence detextors