A Sorted Affair

• Sorting Pre-Flight
  • Checklist
  • Clean Deflection Plates
• Sortware Config
• Drop Charge and Deflection
  • Side Stream alignment
• Find break off point
• Drop Delay calibration
  • Additional Considerations

Sorting Pre-Flight

Checklist

• Supply prep
  • Beads
  • Microscope
  • Slides
• Systems check
  • Drop camera and monitor
  • Sort Chamber
    • Sort stream Illumination
    • Sort chamber laser check
    • Sort chamber door operation

Clean Deflection Plates

Sortware Config

• Sort Settings
• Tray control
• Gating basics
• Sort Modes: Calibration Slides

Drop Charge and Deflection

Side Stream alignment

-   Side stream illumination

• Deflection Plate voltage
• Deflection Percent

Find break off point

• Theory
  • Drop Formation
  • Instrument stability
• Nozzle and Sheath PSI
• Setting frequency (kHz)
• Setting Piezo voltage (V)

Drop Delay calibration

• Calculation via Drop Camera
• Coarse calibration slide
• (Fine) calibration slide

Additional Considerations

• Estimating recovery
• Monitoring the instrument between and during sorts